Validated Electrochemical Methods for the Determination of Antibacterial Veterinary drug Danofloxacin in bulk form and formulation

Two simple, precise, and sensitive voltammetric methods for the determination of Danofloxacin (DFX) were developed. These methods were used to explore the adsorption behavior of the investigated antibacterial through oxidation reduction at hanging mercury dropping electrode (HMDE) by direct method I and secondly by modified method II via its complexation with PdCl2. The drug was accumulated on HMDE and a well defined reduction at pH 7. The adsorption response was evaluated as a function of some variables such as the scan rate, pH and accumulation characters. A linear calibration curves were obtained from 4x10-8 M to 2.4x10-7 M, and from 9x10-9 to 4x10-7 M while the limits of quantification (LOQ) were 1.5x10-7 M, and 6x10-8 M and limits of detection (LOD) were 5.5x10-8 M, and 2x10-8 M for direct HMDE, modified HMDE methods, respectively. The methods were applied to the determination of DFX in bulk and formulation. Complete validation of the proposed methods was also done.